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. 2018 Aug 24;9(66):32580–32592. doi: 10.18632/oncotarget.25953

Figure 4. HELLS knockdown does not affect in osteosarcoma cell proliferation and migration.

Figure 4

(A) Western blot detection of HELLS protein level in osteosarcoma cells after transduction with shRNA against HELLS (shHELLS) compared to non-silencing controls (control) show effective decrease in HELLS protein. (B) Representative images from colony-forming wells with U-2OS cells transduced with shHELLS or non-silencing control. (C) Histogram of the proportion of number of colonies formed in osteosarcoma cells transduced with shHELLS or non-silencing control. Each bar is the mean ± s.d. of triplicate experiments. (D–G) Relative number of shHELLS and control cells over time measured by CellTiter-Glo Luminescence cell viability assay. Each data point is mean ± s.d. of four experiments. (H) Representative image for control (non-silencing control) and shHELLS in 143B cells. (I) Quantification of the distance (pixels) migrated for each of the osteosarcoma cell lines. Each data point is mean ± s.d. of ten measurements in triplicate samples. *p < 0.0332 by two-tailed t test.