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. 2018 Aug 24;9(66):32702–32717. doi: 10.18632/oncotarget.26021

Figure 10. HSP70 inhibitors do not increase the heat shock response.

Figure 10

UMUC3 cells were transfected with a reporter plasmid encoding NanoLuc luciferase driven by a HSP70B’ promoter. (A) Cells were treated with the indicated drug concentrations for 9.5 hours then harvested and assayed for NanoLuc activity. Each treatment was compared using a T-test with a Bonnferroni correction for multiple testing (21 comparisons) p ≤ 0.0024. (B) Cells were treated with the indicated drugs for 1 hour, heat shocked for 30 minutes at 45° C and allowed to recover for 8 hours before being harvested. Each treatment was compared using a T-test with a Bonnferroni correction for multiple testing (21 comparisons) p ≤ 0.0024. (C) Cells were treated with the indicated drugs for 1 hour, then given 1 μM MG132 for 4 hours and harvested. Each treatment was compared using a T-test with a Bonnferroni correction for multiple testing (27 comparisons) p ≤ 0.0018. A comparison of control, non-stressed cells (A), and heat shocked cells (B) is shown in Supplementary Figure 14.