Figure 8. Classification analysis of 6 RPPA-measured proteins in 210 serum samples (132 HCC patients and 78 healthy controls) to evaluate their diagnostic performance as biomarkers of HCC.

(A) The individual ROC curve for each biomarker. (B) Hierarchic clustering of HCC and healthy samples based on expression level changes of all 6 biomarkers. (C) Evaluation of diagnostic performance of all 6 biomarkers against HCC patients (red) and healthy controls (blue) with PCA analysis. (D) AUC rate of the combined 6 biomarkers (B2M, IGFBP2, GP73, GDF15, OPN and AFP) as a specific HCC signature evaluated by supervised LR, LDA, RF and SVM models in a training set and a validation set as described in the Methods section.