Table 5. Validation of antibodies used for RPPA by western-blot (W-B) and ELISA.
| Target | antibody | W-B (pattern of bands) | ELISA (average concentration, n = 40) | RPPA (average concentration, n = 40) | Correlation coefficient between RPPA and ELISA (R,P) |
|---|---|---|---|---|---|
| MMP-9 | MAB9111 | S | 232.22 ng/ml | 24.92 ng/ml | R = 0.936, P < 0.0001* |
| AF911 | M | 232.22 ng/ml | 1485.69 ng/ml | R = –0.71, P = 0.001 | |
| MAB936 | M | 232.22 ng/ml | 862.44 ng/ml | R = 0.582, P = 0.011 | |
| Acrp30 | MAB10651 | M | 89.85 ng/ml | 723.83 ng/ml | R = 0.39, P = 0.401 |
| BAM1065 | M | 89.85 ng/ml | 2.64 ng/ml | R = 0.504, P = 0.092 | |
| AF1065 | S | 89.85 ng/ml | 3144.45 ng/ml | R = 0.971, P < 0.0001* | |
| hVEGF | PB0276B | M | 0.42 ng/ml | 382.37 ng/ml | R = 0.346, P = 0.533 |
| MAB293 | M | 0.42 ng/ml | 0.47 ng/ml | R = 0.691, P = 0.067 | |
| BAF293 | M | 0.42 ng/ml | 1184.23 ng/ml | R = –0.62, P = 0.054 | |
| ApoA | S | 114.07 ug/ml | 1235.67 ug/ml | R = 0.989, P < 0.0001* | |
| ApoE | S | 54.97 ug/ml | 20.59 ug/ml | R = 0.942, P < 0.0001* | |
| A2M | S | 707.94 ng/ml | 4.17 ng/ml | R = 0.746, P = 0.0002* | |
| Clusterin | S | 117.83 | 58.22 | R = 0.176, P = 0.278 | |
| CRP | S | 0.55 | 0.32 | R = 0.769, P = 0.0003* |
S: single band from western-blot; M: multiple bands from western-blot; P < 0.05 means statistically significant; *means good correlation of RPPA with ELISA.