Figure 2.

Expression of Cytokines by hESC-RPE Cells

(A) Real-time PCR analysis of the expression of cytokines by RPE cells cultured for 3 days in the presence or absence of IFN-γ. Relative quantity is the relative expression level of each gene in comparison with its expression level in unstimulated RPE cells that was set at 1.

(B) ELISA analysis of the secretion of cytokines, IL-6, IL-18, and IL-15 by RPE cells cultured for 3 days in the presence or absence of IFN-γ.

(C) RT-PCR analysis of the expression of IL-12A, IL-6, IL-15, and IL-18 by RPE cells cultured for 3 days with or without IFN-γ.

Data are presented as means ± SEM of at least 3 independent experiments. ^∗∗∗p ≤ 0.001.