Figure 3.

T Cell Proliferation Was Highly Attenuated and T Cell Apoptosis Was Enhanced by Co-culture with hESC-RPE Cells

(A and B) Histogram plots presentation of carboxyfluorescein succinimidyl ester (CFSE) fluorescence (A) and histogram presentation of the percentage of dividing CD3⁺ T cells (B). PBMCs were labeled with CFSE and cultured for 4 days with or without RPE cells. Co-culture with RPE cells was either direct or using transwells (trans).

(C) Dot plots presentation of CD3⁺ T cells detected by Annexin V-FITC and PI staining after culture for 1 day with or without RPE cells.

(D) Quantification of the percentage of apoptotic annexin V⁺/PI⁻ T cells following co-culture with RPE. PBMCs were co-cultured with RPE cells directly or using transwells (trans). na, non-activated T cells; act, cells activated with anti-CD3/anti-CD28 antibodies; AnnV, AnnexinV.

Data are presented as means ± SEM of at least 3 independent experiments. ^∗p ≤ 0.05; ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001.