Figure 7.

Increased Jimpy Oligodendrocyte Survival during Differentiation Unmasks a Second Pathological Phase during Myelination

(A) Schematic of the in vitro myelination assay using 4-μm diameter synthetic microfiber fibers detailing the examination of myelinating oligodendrocytes at day 3 and 10.

(B and C) Representative immunocytochemistry images of MBP+ myelinating oligodendrocytes (green), rhodamine+ microfibers (purple), and total DAPI+ cells (blue) at (B) day 3 and (C) day 10. Compound-treated cultures shown at their maximum effective concentration.

(D and E) Quantification of percent total microfiber area overlap with MBP+ myelinating oligodendrocytes at (D) day 3 and (E) day 10 with indicated treatment and genotype. Scale bars, 50 μm. n = 3 replicate wells per compound treatment. n = 16 replicate wells per vehicle control.

Error bars represent mean ± SD. See also Figure S7.