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. 2018 Sep 12;4:93. doi: 10.1038/s41420-018-0095-9

Fig. 3. Inhibition of Kindlin-2 diminishes the pro-fibrogenic activities of TGF-β1.

Fig. 3

a LX-2 cells are transfected with control siRNA or Kindlin-2 siRNA followed by TGF-β1 (10 ng/ml) treatment for 48 h. The expressions of Kindlin-2, Col1A1, and Fn were determined by western blot. b–d After transfection with control siRNA or Kindlin-2 siRNA, LX-2 cells were treated with or without TGF-β1 (10 ng/ml) treatment for 24 h. Real-time PCR analyses of Fn, Col1A1, and α-SMA mRNA were performed. e Immunofluorescence analysis of Fn and α-SMA from LX-2 cells that were transfected with control siRNA or Kindlin-2 siRNA and then treated with or without 10 ng/ml TGF-β1 for 48 h. The data are representative of three independent experiments. Scale bars = 200 μm. *p < 0.05