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. 2018 Sep 12;8:13676. doi: 10.1038/s41598-018-31785-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pharmacological modulation of SIRT1 activity is linked to neurite outgrowth in cultured hippocampal neurons. Hippocampal neurons at DIV3 were treated with vehicle (DMSO) or (a–c) EX527 and (d–f) SRT1720 at the indicated doses for 48 hrs and subjected to immunofluorescent staining for neuron-specific β-III-tubulin. To measure the total neurite length and the length of the longest neurite, fluorescent images obtained with an LSM700 confocal microscope were analyzed using Image-J software. The scale bar represents 50 μm. Values are expressed as (b,e) the total neurite length and (c,f) the length of the longest neurite over the soma size. The bars represent the mean ± S.D. of 35–40 neurons from at least 5 randomly selected areas. ^***p < 0.001; ns, not significant.