Figure 2.
GCM inhibits JNK activation and mitochondria translocation induced by acetaminophen (APAP). C57BL/6N mice were injected with 300 mg·kg−1 acetaminophen, subsequently treated with GCM 50 mg·kg−1 at 2 h and killed at 6 h. Livers were collected to perform the isolation of hepatic mitochondria and cytoplasm. (A–C) Western blot of whole liver homogenate (A), cytoplasm (B) and mitochondria (C) for p‐JNK in mice with or without GCM (50 mg·kg−1). Bar graphs show densitometry of p‐JNK/JNK or p‐JNK/PHB1. (D) Western blot of mitochondrial fraction for Drp1 and densitometry. Quantitative data are presented as mean ± SD for n = 6 mice per group. *P < 0.05 compared with the corresponding acetaminophen group. PHB1, prohibitin 1.