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. 2018 Sep 12;8:13685. doi: 10.1038/s41598-018-31638-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of red and white light on singlet oxygen imaging in Arabidopsis leaves. Arabidopsis leaves were treated in 50 μM SOSG for 30 min either in dark or exposed to red/white light. For each treatment following images are presented (from left to right): Nomarski DIC, SOSG-EP fluorescence (λ_em = 505–525 nm), combined channel and integral distribution of the signal intensity within the sample (Z-axis represents the levels of brightness for each pixel, ranging between 0 and 3200). Bar represents 20 µm.