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. 2018 Sep 12;8:13685. doi: 10.1038/s41598-018-31638-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of mild heating on the penetration of SOSG into the Synechocystis cells. Synechocystis cells were exposed to red light for 30 min in the presence of 50 μM SOSG at the temperature (25 °C) (upper panel) and (37 °C) (lower panel). From left to right are Nomarski DIC, SOSG-EP fluorescence (λem = 505–525 nm) and combined channel (DIC + SOSG-EP). Bar represents 5 µm.