Fig. 5. Role of Bak/Bax in macrophage and NO-induced apoptosis.

a [³H]-thymidine-labeled BMK-E1A, BMK-E1A-Bak^−/−, BMK-E1A-Bax^−/−, or BMK-E1A-Bak/Bax^−/−/^−/− cells were incubated with ceramide (100 µM) overnight. Supernatants were collected and % specific thymidine release was assessed (mean ± SEM; n = 5, ^***P = 0.001, one-way ANOVA). b [³H]-thymidine-labeled BMK, BMK-E1A, BMK-E1A-Bak^−/−, BMK-E1A-Bax^−/−, or BMK-E1A-Bak/Bax^−/−/^−/− cells were incubated with activated bone marrow-derived macrophages at E:T of 30:1 in the absence or presence of 100 μM zVAD-fmk. After 48 h supernatants were collected and % specific thymidine release was assessed (mean ± SEM; n = 3, ^***P ≤ 0.0001, ^**P = 0.029, one-way ANOVA). c [³H]-thymidine-labeled BMK, BMK-E1A, or BMK-E1A-Bak/Bax^−/−/^−/− cells were incubated with DETA-NONOate at 650 µM alone (black bars), with 100 μM zVAD-fmk (white bars) or with cyclosporine A (100 µM) (CSA, hatched bars). After 18 h supernatants were collected and % specific thymidine release was assessed (mean ± SEM; n = 3, ^***P ≤ 0.0005, ^**P = 0.01, one-way ANOVA)