Fig. 3.
APRIL together with the MSC secretome enhances blood ASC survival. a, b Blood ASC survival is enhanced by the MSC secretome with exogenous APRIL. First two rows: sorted blood ASC were cultured in R10 alone or in media with exogenous APRIL for 7 days. Second two rows: blood ASC were cultured in MSC secretome alone or the secretome with APRIL. Blood ASC immediately from FAC sorting are shown (day 0). Representative images of Elispot wells are shown. Graphic plot of % survival of IgG ASC in media alone (R10) (open circles), APRIL alone (open squares), secretome alone (green circles), or secretome + APRIL (red triangles) (p < 10-6; liner regression modeling between secretome alone and secretome + APRIL). c Exogenous APRIL together with the MSC secretome enhances ASC survival in short-term cultures. Blood ASC from four different subjects after vaccination were cultured in media alone (open circles), secretome alone (green circles), or secretome + APRIL (red triangles). Percentage of IgG Elipsots normalized to maximal frequency on days 1–3. Shown are p-values between secretome alone & secretome + APRIL. d Exogenous APRIL with the MSC secretome enhances ASC survival in long-term cultures. ASC cultured with the MSC secretome (green circles) or MSC secretome + APRIL (red triangles). e Treatment of MSC secretome with anti-IL-6 antibodies (anti-IL-6) diminishes blood ASC survival. Blood ASC were cultured in MSC secretome or MSC secretome treated with anti-IL-6 for 1, 3, 7 days and IgG Elispots were performed (p < 10−5; linear regression modeling between secretome vs secretome + anti-IL-6). The frequency of isotype controls was similar to MSC secretome. Representative of three experiments