Fig. 6.

Survival of blood ASC is enhanced under hypoxic conditions with the MSC secretome and APRIL. a Circulating ASC post-tetanus vaccination from one healthy adult were cultured under the following conditions in normoxia: media alone (black open squares), secretome alone (open red squares), or secretome + APRIL (red closed squares) and under hypoxia conditions: media alone (black open circles), secretome alone (blue circles), or secretome + APRIL (blue closed circles). Percentage of IgG Elispots were normalized to the maximal frequency on day 3. Data are representative of four different blood ASC from four adults after vaccination (p-values shown in the table below). b Representative images of Elispot wells from the experiments in a are shown. Number of ASC per well: 1333 except on wells from days 21–56 for MSC secretome + APRIL in normoxia or hypoxia (800) to show images in a countable range