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. 2018 Sep 12;8:13689. doi: 10.1038/s41598-018-32019-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the two different trials (in vivo challenge with Neoparamoeba perurans and field sampling) used in this study. SD = initial stocking density. Black dots represent the sequential sampling points (dpi = days post-infection; dpt = days post-transfer). Blue arrows in the field sampling section show initial sea transfer and posterior freshwater treatment. Details on type of lethal samples taken are described in the material and methods section.