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. 2018 Sep 12;8:13689. doi: 10.1038/s41598-018-32019-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCA analysis for the in vivo challenge samples representing the distribution of infected samples at 2, 7, 14 and 21 dpi. Analysis was based on fold-changes of all genes for each individual sample (smaller symbols) relative to the negative control at each time point. The ellipses indicate the group dispersion/variability from the centroid (larger symbols) calculated using all individual fold-changes values/group.