Table 2.

EC50 values from the Titration ELISA of JoJ48C11 scFv-Fc and its point mutants.

scFv-Fc	EC50 [nM]	scFv-Fc	EC50 [nM]
JoJ48C11	0.22 ± 0.01	Glu59AlaH	0.52 ± 0.03
Trp27AlaH	0.86 ± 0.11	Trp100AlaH	10.94 ± 1.15
Ser31AlaH	0.22 ± 0.04	Asp105AlaH	>>1000
Trp33AlaH	0.36 ± 0.08	Asp49AlaL	163.2 ± 9.5
His35AlaH	2.28 ± 0.17	Lys52AlaL	4.78 ± 0.26
Trp47AlaH	6.40 ± 1.17	Ser55AlaL	0.30 ± 0.06
Tyr50AlaH	0.30 ± 0.05	Trp90AlaL	1.07 ± 0.09
Tyr57AlaH	0.22 ± 0.02	Asn93AlaL	0.23 ± 0.02