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. 2018 Sep 12;8:13654. doi: 10.1038/s41598-018-31856-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CryoEM maps and atomic models of the chimeric viruses, (a) a whole virus map of SAT2 wildtype particle. A single protomer is highlighted and coloured as VP1 – blue, VP2 – green and VP3 – red. The inset shows the zoomed-up density of the VP2 alpha-helix at the icosahedral 2-fold axis with a fitted atomic model. The side chain for VP2 residue 93S is shown in stick representation in cyan. (b) Atomic model for SAT2 wildtype. Colouring reflects the different viral proteins, VP1 (blue), VP2 (green), VP3 (red) and VP4 (yellow). The VP1 GH loop is in the down conformation, the associated EM density is shown in grey. (c) and (d) EM electric potential map and atomic model of SAT2/O, depicted as (a) and (b) respectively. (e) and (f) EM electric potential map and associated atomic model of SAT2/O mut2, depicted as (a) and (b) respectively. (g) and (h) EM electric potential map and associated atomic model of ts-SAT2/O, depicted as (a) and (b) respectively. The inset shows the tyrosine sidechain making predicted stacking interactions at the 2-fold axis at position VP2 93. The VP1 GH loop is found to be flexible and hence no density is visible in the EM map.