Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 12;8:13712. doi: 10.1038/s41598-018-32009-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HDAC1 associates with components of the prefoldin-mediated CCT folding pathway. (A) The established mechanism for prefoldin delivery of client proteins to the CCT complex^37,44,65 used to model possible HDAC1 processing by the prefoldin/CCT pathway in the nucleus. (B) Prefoldin subunit VBP1 interacts with HDAC1. HEK293T cell lysates expressing tagged VBP1 with or without Halo-tagged versions of HDAC1 were used for Halo affinity purification. Samples were analysed by SDS PAGE followed by Western blotting or Coomassie staining. SNAP-FLAG-VBP1 was detected using anti-FLAG mouse monoclonal primary antibody and IRDye® 680LT labeled goat anti-Mouse secondary antibody. Halo-tagged HDAC1 was detected using anti-HDAC1 rabbit polyclonal primary antibody and IRDye® 800CW labeled goat anti-Rabbit secondary antibody. Full length images are presented in Supplementary Figure 3. (C) VBP1 colocalises with HDAC1 in the nucleus. Flp-In™-293 host cells stably expressing Halo-HDAC1 were transiently transfected with SNAP-FLAG-VBP1 and proteins imaged using HaloTag® TMRDirect™ ligand (Halo-HDAC1; red), SNAP-Cell® 505-Star ligand (SNAP-FLAG-VBP1; green), and Hoechst dye (nuclei; blue). (D) TCP1 and other CCT complex components interact with HDAC1. The STRING³⁸ network showing established TCP1 (CCT complex) interacting proteins was generated using the following settings: minimum required interaction score - medium confidence (0.4); maximum number of interactors - 50; active interaction source – Experiments; clustering method – kmeans (default settings). Clusters are indicated by node color.