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. 2018 Sep 12;50(9):121. doi: 10.1038/s12276-018-0146-6

Fig. 1. β-Elemene inhibited growth in lung cancer cells.

Fig. 1

a H1975 cells were stimulated with different concentrations of β-elemene for up to 72 h. The cells were collected and processed for MTT assay, as described in the Materials and Methods section. b A549 and H1975 cells were treated with β-elemene (25 μg/mL) for 48 h followed by determination of cell growth with the Cell-Light EdU DNA cell proliferation kit. The image was magnified ×10. Hoechst was used to stain all nuclei. At least 3 captured fields were randomly selected, and the percentage of EdU-positive cells = (EdU-positive cells/Hoechst stain cells) × 100. c A549 and H1975 cells were treated with β-elemene (30 μg/mL) for 24 h, and the protein levels of Bax were measured by Western blot analysis. GAPDH was used as loading control. Values and bar graphs are presented as the mean ± SEM or the mean ± SD of Bax/GAPDH of 3 independent experiments. *indicates significant difference from the control group (P < 0.05). **indicates significant difference between combination treatment and treatment with β-elemene alone (P < 0.05)