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. 2018 Sep 12;50(9):121. doi: 10.1038/s12276-018-0146-6

Fig. 2. β-Elemene decreased phosphorylation of Stat3 and increased protein expression of FOXO3a via Stat3.

Fig. 2

a A549 and H1975 cells were exposed to the increased concentration of β-elemene for 24 h, and the phosphorylation and protein expression of Stat3 were measured by western blot analysis. GAPDH was used as loading control. The bar graphs represent the mean ± SD of p-Stat3/GAPDH of 3 independent experiments. b A549 and H1975 cells were exposed to the increased concentration of β-elemene for 24 h, and the protein expression of FOXO3a was measured by western blot analysis. GAPDH was used as loading control. The bar graphs represent the mean ± SD of FOXO3a/GAPDH of 3 independent experiments. c A549 and H1975 cells were transfected with the control or Stat3a expression vectors for 24 h and then exposed to β-elemene (25 μg/mL) for an additional period of 24 h. The phosphorylation and protein expression levels of Stat3 and FOXO3a were subsequently examined by western blot analysis. GAPDH was used as loading control. The bar graphs represent the mean ± SD of FOXO3a/GAPDH of 3 independent experiments. *indicates significant difference compared with the untreated control group (P < 0.05); **indicates significant difference between combination treatment and treatment with β-elemene alone (P < 0.05)