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. 2018 Sep 12;8:13701. doi: 10.1038/s41598-018-32065-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Elevated phosphaturia generates renal oxidative stress. Glutathione peroxidase (GPx) activity in renal tissue. (A) GPx activity was determined in 20 µg of total protein extracts of kidney samples by a coupled reaction with glutathione reductase. Units are expressed as nanomoles of NADPH oxidized per minute and milliliter. White bars are LPD (0.2%P diet) groups and black bars are HPD (1.2%P diet) groups. t-test analysis *p < 0.05, **p < 0.01. (B) Negative correlation between renal mRNA α-klotho expression and GPx activity.