Fig. 1.

Investigation of CD123 expression in high-risk MDS. a Mass cytometry analysis of a representative high-risk MDS bone marrow specimen. The SPADE algorithm was used to cluster independent populations based on the expression of 14 independent cell surface markers. Blue to Red heat map coloring indicates relative expression of CD34 within each of the subpopulations identified by SPADE analysis. The red boxed cluster indicates all detected subpopulations defined as CD34+/Lin−. b Detailed analysis of CD34+ nodes. Indicated by red outline is the CD34+, CD38-, Lin- nodes. Heat map coloring indicates relative expression of CD123. c, d Flow cytometric analysis of CD34+/CD38−/Lin− MDS bone marrow cells labeled with CD123. Panel c shows comparison of representative low vs. high-risk specimens. Panel d shows additional high-risk MDS specimens in comparison to an isotype control. e Aggregate data of CD123 mean fluorescent intensity in high-risk vs. low-risk MDS patient samples showing a significant increase in high-risk MDS. See supplementary figure 1A for analysis of additional specimens. * p < 0.05 (two-tail t-test) error bars are S.D