Fig. 2.

Whole-transcriptome analysis shows an upregulation of ribosome signaling in CD123+ MDS cells. RNA-seq was performed on Lin−/CD34+/CD38−/CD123+ or Lin−/CD34+/CD38−/CD123− primary MDS BM cells from three independent patients. a Heat map shows the top 50 up and downregulated genes. b Using Enrichment map algorithm, top pathways and groups of pathways were delineated in CD123+ vs. CD123− cells. c–f Using the KEGG database, the ribosome signature was the most significantly enriched gene set in the translation cluster of nodes in CD123+ cells (panel b). d The Regulation of IFNA Signaling gene set was also highly enriched in IL1 signaling group of nodes in CD123+ cells. e The Regulation of Chromosome Segregation was highly enriched in the mitotic events cluster of nodes in CD123- cells. f The Heme Biosynthetic Process pathway was highly enriched in the Heme signaling pathway in CD123- cells