Fig. 4.

CD123 positive cells have a distinct metabolic phenotype. HPLC-based metabolomic analysis was done on sorted Lin−/CD34+/CD123+ cells from two high-risk MDS patients. a Principle component analysis of all measured metabolites shows similarities between CD123+ MDS stem cells from both patients (green circle). Bulk lineage negative cells (red circle) show distinctly different profiles. b Pathways most enriched in CD123+ MDS cells compared to CD123- population as determined by Metaboanalyst software analysis of metabolomics data. c Heat map representation of major metabolite differences including ATP, Glutamate, and TCA cycle intermediates. Specimen analyzed in triplicate (derived from patient 4—Supplementary Table 3). d Oxidative state analysis as indicated by CellROX dye labeling. e Aggregate data of CellROX labeling shows no significant difference between CD123+ vs. CD123− cells