Table 2.
One-site | Two-site | Hill slope | F | Preferred model | |||
---|---|---|---|---|---|---|---|
Ki (nM) | Ki1 (nM) | Ki2 (nM) | |||||
Rolipram | Wild-type | 1.99 ± 0.11 | 0.75 ± 0.33 | 34.1 ± 26.83 | −0.75 ± 0.08 | 2.92 | One-site |
hPDE4D | 1.66 ± 0.07 | 0.39 ± 0.07 | 6.10 ± 0.79 | −0.71 ± 0.02 | 1.91 | One-site | |
BPN14770 | Wild-type | 124.4 ± 14.1 | 79.7 ± 17.09 | 622.4 ± 109.8 | −0.89 ± 0.06 | 0.98 | One-site |
hPDE4D | 82.1 ± 6.03 | 2.80 ± 1.08 | 186.3 ± 19.0 | −0.56 ± 0.02 | 11.25 | Two-site |
Ki values, Hill slope, and F-ratio were calculated by non-linear regression. Binding to a single site was assumed unless the data were better described by a two-site model, which is characterized by a small Hill slope and large residual sum of square (F-ratio). Statistical difference was set at p < 0.001. Data shown are from three independent experiments (mean ± SEM)