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. 2018 Aug 14;43(11):2299–2309. doi: 10.1038/s41386-018-0178-6

Table 2.

Inhibition of [3H]-rolipram binding to mouse brain membranes by BPN14770 or rolipram

One-site Two-site Hill slope F Preferred model
Ki (nM) Ki1 (nM) Ki2 (nM)
Rolipram Wild-type 1.99 ± 0.11 0.75 ± 0.33 34.1 ± 26.83 −0.75 ± 0.08 2.92 One-site
hPDE4D 1.66 ± 0.07 0.39 ± 0.07 6.10 ± 0.79 −0.71 ± 0.02 1.91 One-site
BPN14770 Wild-type 124.4 ± 14.1 79.7 ± 17.09 622.4 ± 109.8 −0.89 ± 0.06 0.98 One-site
hPDE4D 82.1 ± 6.03 2.80 ± 1.08 186.3 ± 19.0 −0.56 ± 0.02 11.25 Two-site

Ki values, Hill slope, and F-ratio were calculated by non-linear regression. Binding to a single site was assumed unless the data were better described by a two-site model, which is characterized by a small Hill slope and large residual sum of square (F-ratio). Statistical difference was set at p < 0.001. Data shown are from three independent experiments (mean ± SEM)