Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 12;8:13713. doi: 10.1038/s41598-018-32054-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HIF2α stabilization results in exocrine cell atrophy and expansion of duct-like tubular structures. (A) Body weight (left panel), pancreas weight (middle panel) and body/pancreas weight ratio (right panel) in Pdx1-Cre;HIF2dPA and control mice at 2 and 8 weeks of age. Data are presented as mean ± SD. (B) HIF2α accumulation in Pdx1-Cre;HIF2dPA analyzed by Western blot with anti-HA antibody. Two independent two-week-old control and mutant mice are shown. ß-actin protein was used for loading control. Full-length blots are presented in Supplementary Fig. 2. (D) Immunofluorescence analysis of HIF2α in two-week-old control pancreata. Endogenous HIF2α expression is observed in islets (marked by an white asterisk) but not in exocrine tissue. (D) Robust HIF2α accumulation in the pancreas of two-week-old Pdx1-Cre;HIF2dPA mice. Hematoxylin/Eosin-stained pancreatic sections from P0 (E,F), two- (I,J) and eight-week-old (M,N) Pdx1-Cre;HIF2dPA and control mice. Inset in N shows an area with adipose tissue in Pdx1-Cre;HIF2dPA pancreata. Immunofluorescence of amylase and KRT19 shows no differences between Pdx1-Cre;HIF2dPA and control mice at P0 (G,H). Duct-like tubular structures and loss of amylase immunoreactivity in two- (K,L) and eight-week-old (O,P) Pdx1-Cre;HIF2dPA mice compared to control mice. Note areas with normal acini in 8-week-old Pdx1-Cre;HIF2dPA mice (white asterisk in O). Insets in (H,L and P) show higher magnification pictures. DAPI staining is shown in blue in (C,D,G,H,K,L,O and P). Scale bars = 50 µm for (C,D); 100 µm for (E–P). ***P < 0.001.