Table 2.
Primers specification.
| Gene | Accession number | Forward | Revers | Efficiency | Amplicon length (bp) |
|---|---|---|---|---|---|
| Agrn | NM_175754 | TACCTGTCCACTTGTATT | TTCTCATCCAATAACACATT | 98.5 | 87 |
| Arhgap4 | NM_144740 | CTTGTGAGCCATCTACTATC | GTTGAGGAAGGTGAAGAG | 88 | 75 |
| Anxa2 | NM_019905 | CTACTGTCCACGAAATCCTG | AAGTTGGTGTAGGGTTTGAC | 99.8 | 94 |
| Casp6 | NM_031775 | ACAGATGGCTTCTACAGA | AGTTCCTCTCCTCTTGTG | 102.2 | 78 |
| Chmp1a | NM_001083313 | ATCAACTTACAGGTTAGG | TACTTACGACAACATTCTA | 98.2 | 122 |
| Col4a3 | NM_001135759 | TCACCACAATGCCATTCTTA | CGACAGCCAGTATGAATAGT | 94.5 | 83 |
| Icam1 | NM_012967 | ACCTACATACATTCCTACC | ATGAGACTCCATTGTTGA | 96.3 | 91 |
| Mag | NM_017190 | ACCATCCAACCTTCTGTATC | CTGATTCCGCTCCAAGTG | 96.2 | 90 |
| Ptk2b | NM_017318 | TGTCTACACGAACCATAA | GAACTTCTCCTTGTTGTC | 93.1 | 88 |
| Tubb2b | NM_001013886 | CAGTTGGAAGAAGGAGAA | AGTGTTACATTGATGTTATCG | 107.5 | 111 |
| Il6 | NM_012589.1 | GCCCACCAGGAACGAAAGTC | TCCTCTGTGAAGTCTCCTCTCC | 107.7 | 161 |
| Hprt | NM_012583.2 | AGACTGAAGAGCTACTGTAATGAC | GGCTGTACTGCTTGACCAAG | 94.9 | 163 |