Figure 6.
hESC-Derived Progenitors Survive and Produce MSNs in QA-Lesioned Mice
(A) Experimental scheme for the transplantation of QA-lesioned mice and behavior tests.
(B) Microtome sections of QA-lesioned adult mouse brains at 4, 8, and 16 weeks post transplantation were analyzed by immunohistochemistry for hN and NESTIN or KI67. Scale bar, 50 μm.
(C) Adult SCID mice injected with QA and without cell transplantation showed a loss of neurons marked by NEUN staining and MSNs marked by DARPP32 staining on the lesion side (ctrl-ipsil), while normal on contralateral side of the striatum (ctrl-contral). Scale bar, 50 μm.
(D) Low magnification of a coronal brain section showing transplanted human cells labeled with hN residing in the striatum. Scale bar, 1,000 μm.
(E) Immunostaining images of 16-week-old grafts for GABA, DARPP32, TUJ1, FOXP1, substance P (SP), and enkephalin (Enk). Scale bar, 50 μm.
(F) Triple staining for DARPP32, CTIP2, and hN on a section of a 16-week-old grafts. Scale bar, 50 μm.
(G) Quantification of immunostaining of striatal markers, shown as a percentage of hN+ cells. Data are presented as means ± SEM.
(H) Representative tracing images of animals in open field tests.
(I) Open field tests indicated increased total distances in animals receiving cell grafts compared with sham control. Open field behaviors were analyzed by two-way ANOVA (∗∗∗p < 0.001; control group, n = 13; cell group, n = 13).
(J) Rotarod tests showed increased latency in animals transplanted with hESC-derived progenitors compared with sham control. The tests were analyzed by two-way ANOVA (∗∗p < 0.01, ∗∗∗∗p < 0.001; control group, n = 13; cell group, n = 13).
See also Figures S3–S5.