Figure 4.

Short-Lived Reporter Dynamics in Aging Cells

(A and B) Mean ssGFP intensity, a measure of protein concentration, over generations for cells aligned to birth (A) or death (B).

(C and D) Whole-cell ssGFP intensity, a measure of protein abundance, over generations for cells aligned to birth (C) or death (D).

(E and F) Intercellular variability, defined as the SD divided by the mean for mean ssGFP intensity across measurements of all cells in the population at each generation. In (E), cells are aligned at birth, and in (F), cells are aligned at death.

(G and H) Mean intracellular variability, defined as the SD/mean of mean ssGFP intensity for 8 contiguous generations, over generations for cells aligned to birth (G) or death (H). All data represent measurements from a single experiment with ssGFP integrated in a heterozygous fashion at the SAM2 locus under the P_TEF1 promoter. N = 50 cells for all subfigures, with data points where less than 10 cells remained alive omitted. Error bars are SEM.

See also Figures S2 and S7, and Tables S2–S4.