Figure 1.

RBC adhesion to brain endothelial (bEND3 cells) under different conditions (A) and PS exposure in t-BHP-treated RBC (B). RBC treated with PBS (control), LPS (10 μg/mL) or neuraminidase (0.125 U or 0.5 U) did not significantly alter RBC adhesion to bEND3 cells following 3 h incubation, however, 3 mM t-BHP treatment of RBC resulted in a significant increase in RBC adhesion to bEND3 cells compared with respective PBS-treated RBC. LPS treatment of bEND3 cells did not further enhance RBC adhesion under any treatment conditions. >90% of 0.3 mM t-BHP-treated RBC were PS positive compared with PBS-treated RBC as determined by flow cytometry (B). Data is presented as mean ± SEM of at least 3 independent experiments. ***p < 0.001.