Figure 4.

Transmigration of hemoglobin across the bEND3 monolayer. Experimental set up to detect passage of hemoglobin across the bEND3 monolayer (A). Significant increase in the passage of hemoglobin across the bEND3 monolayer incubated with t-BHP-treated RBC compared with PBS-RBC. Spontaneous migration reflects passage of hemoglobin from PBS-RBC across the 3 μm pore size transwell in the absence of a bEND3 monolayer (B). Passage of hemoglobin across the bEND3 monolayer grown on transwells incubated with t-BHP-RBC was not accompanied by a change in TEER; TEER measurements are presented as % of PBS-RBC values (C). Free hemoglobin released by t-BHP-treated RBC is significantly lower compared with PBS-treated RBC. The bEND3 monolayer was grown on standard cell culture wells (D). Data is presented as mean ± SEM of at least 3 independent experiments. **p < 0.01, ****p < 0.0001.