Figure 3.

Real-time PCR quantification of expression of genes involved in immune responses in zebrafish kidneys. Data are presented as ΔCT (delta cycle threshold, expression normalized to housekeeping gene EF1) using box and whisker plots. Expression of genes before and after challenge is represented by solid colored and pattern-filled boxes, respectively (n = 4 biological replicates, a pool of five fish each). Significant difference between treatments before and after challenge is indicated in bracket with corresponding p value, as determined by t-test. A and B denote significant differences between treatments before challenge; a and b denote significant differences between treatments after challenge. Differences were considered significant at p < 0.05.