Table 4.
Fatty acid composition (% of TFA) and content in liver neutral lipids of zebrafish fed with a diet supplemented (w/w) with different levels (7.5 and 15%) of the microalga Lobosphaera incisa WT and mutant P127.
| Fatty acid | Before challenge | After challenge | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| WT 7.5 % | WT 15 % | MUT 7.5% | MUT 15% | Control | WT 7.5 % | WT 15 % | MUT 7.5% | MUT 15% | Control | |
| 14:0 | 1.8 ± 0.3 | 1.9 ± 0.2 | 1.8 ± 0.1 | 1.8 ± 0.1 | 1.8 ± 0.2 | 2.1 ± 0.1 | 1.2 ± 0.8 | 1.4 ± 0.9 | 2.3 ± 1.1 | 1.8 ± 0.4 |
| 16:0 | 22 ± 1.3 | 21.4 ± 0.2 | 20.7 ± 0.5 | 20.4 ± 0.8 | 22.6 ± 1.0 | 30.2 ± 1.8 | 24.9 ± 3.1 | 23.8 ± 0.1 | 21.2 ± 3.8 | 24.4 ± 2.6 |
| 16:1n-5 | 0.8 ± 0.1 | 0.7 ± 0.1 | 0.9 ± 0.3 | 0.7 ± 0.1 | 0.8 ± 0.2 | 0.3 ± 0.5 | 0.4 ± 0.4 | 0.5 ± 0.4 | 0.6 ± 1.6 | 1.3 ± 2.1 |
| 16:1n-7 | 3.6 ± 0.4 | 3.6 ± 0.5 | 3.2 ± 0.2 | 3.2 ± 0.2 | 3.9 ± 0.2 | 2.9 ± 2.5 | 2.6 ± 1.9 | 2.2 ± 1.4 | 3.7 ± 0.4 | 2.9 ± 2.7 |
| 16:2 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.2 | 0.3 ± 0.1 | 0.3 ± 0.2 | 0.4 ± 0 | 0.4 ± 0.4 | 0.4 ± 0.2 | 0.5 ± 0 | 0.3 ± 0.3 |
| 16:2 | 0.5 ± 0.2 | 0.5 ± 0.2 | 0.8 ± 0.1 | 0.5 ± 0.4 | 0.5 ± 0.1 | 2.5 ± 2.6 | 0.5 ± 0.2 | 2.7 ± 4.1 | 0.6 ± 0.7 | 1.7 ± 1.1 |
| 16:3 | 0.7 ± 0.3 | 0.7 ± 0.1 | 0.6 ± 0.1 | 0.5 ± 0 | 0.6 ± 0.1 | 0.6 ± 0.3 | 0.8 ± 0.6 | 0.6 ± 0.4 | 0.6 ± 0.3 | 1.0 ± 0.7 |
| 18:0 | 4.3 ± 0.4 | 4.2 ± 0.1 | 4.8 ± 0.8 | 4.5 ± 0.4 | 4.4 ± 0.2 | 9.1 ± 1.4 | 8.7 ± 1.8 | 13.0 ± 11.1 | 4.1 ± 7.1 | 10.6 ± 7.2 |
| 18:1n-9 | 27.2 ± 1 | 27.4 ± 1.5 | 29.1 ± 2.8 | 30.5 ± 0.6 | 30.1 ± 1.0 | 25 ± 4.0 | 26.4 ± 1 | 18.4 ± 9.4 | 29 ± 6.0 | 24.9 ± 9.1 |
| 18:1n-7 | 3.0 ± 0.2 | 3.0 ± 0.1 | 2.8 ± 0.2 | 2.7 ± 0.2 | 2.9 ± 0.3 | 2.9 ± 0.1 | 2.7 ± 1.1 | 2.6 ± 1.3 | 3.4 ± 0.1 | 2.5 ± 1.0 |
| 18:2n-6 | 15.5 ± 0.6 | 15 ± 0.9 | 15.1 ± 1.9 | 15 ± 1.3 | 13.8 ± 1.1 | 8.9 ± 0.1 | 10.8 ± 4.1 | 8.1 ± 5.4 | 14.5 ± 1.7 | 11.2 ± 2 |
| 18:3n-6 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.1 | 0.4 ± 0.0 | 0.3 ± 0.0 | 0.1 ± 0.1 | 0.6 ± 0.6 | 0.1 ± 0.1 | 0.4 ± 0.2 | 0.2 ± 0.1 |
| 18:3n-3 | 2.1 ± 0.3 | 2.0 ± 0.2 | 2.0 ± 0.2 | 2.0 ± 0.5 | 2.0 ± 0.6 | 0.7 ± 0.1 | 1.2 ± 1.0 | 1.3 ± 1.0 | 2.4 ± 0.6 | 1.4 ± 1.6 |
| 18:4n-3 | 0.8 ± 0.1 | 0.8 ± 0.1 | 0.9 ± 0 | 0.8 ± 0.2 | 0.7 ± 0.1 | 0.8 ± 0.0 | 0.8 ± 0.4 | 0.7 ± 0.3 | 0.9 ± 0.3 | 0.6 ± 0.4 |
| 20:0 | 0.4 ± 0.1 | 0.4 ± 0.2 | 1.2 ± 0.6 | 0.3 ± 0.1 | 0.3 ± 0.2 | 0.2 ± 0.1 | 0.5 ± 0.5 | 0.1 ± 0.2 | 0.2 ± 0.3 | 0.1 ± 0.1 |
| 20:1 | 2.3 ± 0.1 | 2.3 ± 0.2 | 2.1 ± 0.2 | 2.2 ± 0.2 | 2.4 ± 0.2 | 1.6 ± 0.1 | 2.4 ± 2.8 | 2.0 ± 1.1 | 2.2 ± 0 | 2.3 ± 0.4 |
| 20:2 | 0.5 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.1 | 0.5 ± 0.2 | 1.4 ± 0.4 | 0.3 ± 0.3 | 0.5 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.2 |
| 20:3n-6 (DGLA) | 0.5 ± 0.2c | 0.5 ± 0.1c | 1.4 ± 0.3b | 2.8 ± 0.4a | 0.5 ± 0.3c | 0.5 ± 0.1 | 0.6 ± 0.3 | 1.3 ± 1.3 | 2.6 ± 0.4 | 1.1 ± 0.7 |
| 20:4n-6 (ARA) | 2.1 ± 0.4c | 3.3 ± 0.2a | 0.7 ± 0.5b | 0.6 ± 0.2b | 0.4 ± 0.2b | 1 ± 0.4 | 2.1 ± 0.8 | 2.2 ± 2.1 | 1.8 ± 0.4 | 1.1 ± 0.8 |
| 20:4n-3 | 0.5 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.0 | 0.5 ± 0.1 | 0.2 ± 0.0 | 0.2 ± 0.2 | 0.1 ± 0.2 | 0.4 ± 0.7 | 0.4 ± 0.3 |
| 20:5n-3 (EPA) | 2.6 ± 0.3 | 2.4 ± 0.4 | 2.5 ± 0.1 | 2.5 ± 0.3 | 2.5 ± 0.5 | 0.7 ± 0.1 | 2 ± 0.3 | 1.2 ± 1.2 | 2.4 ± 0.4 | 2.1 ± 0.9 |
| 22:0 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.6 ± 0.4 | 0.2 ± 0 | 0.3 ± 0.1 | 0.6 ± 1.0 | 0.7 ± 1.0 | 0.1 ± 0.2 | 0.0 ± 0.0 | 0.1 ± 0.1 |
| 22:4n-6 | 0.2 ± 0.1 | 0.2 ± 0 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.1 | 1.3 ± 2.2 | 0.7 ± 0.7 | 0.6 ± 1.0 | 0.2 ± 0.7 | 0.3 ± 0.2 |
| 22:5n-6 | 0.2 ± 0.1 | 0.2 ± 0.2 | 0.4 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.2 | 0.4 ± 0.4 | 0.1 ± 0 | 0.5 ± 0.2 | 0.3 ± 0.5 | 0.2 ± 0.2 |
| 22:5n-3 | 0.8 ± 0.1 | 0.7 ± 0 | 0.7 ± 0.1 | 0.6 ± 0.1 | 0.7 ± 0.1 | 1.3 ± 1.9 | 0.5 ± 0.4 | 0.9 ± 0.7 | 0.6 ± 1.5 | 0.8 ± 0.3 |
| 22:6n-3 | 4.2 ± 0.6 | 4.1 ± 0.1 | 4.2 ± 0.4 | 4.0 ± 0.3 | 4.3 ± 0.6 | 1.9 ± 0.5 | 3.7 ± 0.9 | 2.4 ± 2.1 | 3.4 ± 0 | 3.6 ± 1.8 |
| FA content (μg mg−1 DW)* | ||||||||||
| DGLA | 0.3 ± 0.1b | 0.4 ± 0.2b | 0.9 ± 0.4ab | 1.8 ± 1.2a | 0.4 ± 0.2b | 0.3 ± 0.2b | 0.2 ± 0.1b | 0.3 ± 0.2b | 1.4 ± 1.0a | 0.2 ± 0.1b |
| ARA | 1.6 ± 0.3ab | 2.6 ± 1.8a | 0.5 ± 0.6b | 0.4 ± 0.2b | 0.3 ± 0.2b | 0.5 ± 0.3b | 0.9 ± 0.2b | 0.7 ± 0.2a | 0.9 ± 0.1ab | 0.3 ± 0.2b |
| TFA | 77.4 ± 26.7 | 78.4 ± 52.4 | 66.4 ± 32.1 | 67.1 ± 51.7 | 91.3 ± 68.4 | 43.8 ± 19.6 | 43.7 ± 12.2 | 26.4 ± 22.1 | 50.2 ± 15.7 | 28.3 ± 22.5 |
Data represents fatty acid composition from the neutral lipid fraction before and after S. iniae challenge. Values shown as mean ± SD, n = 20. Different letters a, b and c denote significant (p < 0.05) difference in treatments, as determined by ANOVA.
*
FA content represents μg of the FA per mg of liver dry weight (DW).