Table 5.
Fatty acid composition (% of TFA) and content in liver polar lipids of zebrafish fed with diets supplemented (w/w) with different levels (7.5 and 10%) of the microalga Lobosphaera incisa WT and mutant P127.
| Fatty acid | Before challenge | After challenge | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| WT 7.5 % | WT 15 % | MUT 7.5% | MUT 15% | Control | WT 7.5 % | WT 15 % | MUT 7.5% | MUT 15% | Control | |
| 14:0 | 1.0 ± 0.3 | 0.7 ± 0.1 | 0.8 ± 0.2 | 0.9 ± 0.3 | 0.9 ± 0.2 | 0.7 ± 0.2 | 0.4 ± 0 | 1.7 ± 0.3 | 1.6 ± 0.2 | 1.5 ± 0.5 |
| 16:0 | 21.3 ± 0.9 | 21.5 ± 0.4 | 21.9 ± 0.3 | 21.7 ± 2.2 | 21.8 ± 0.8 | 22.0 ± 1.1 | 21.2 ± 0.3 | 25.0 ± 7.1 | 26.1 ± 11.9 | 24.7 ± 3.0 |
| 16:1n-5 | 0.9 ± 0.7 | 0.4 ± 0 | 0.6 ± 0.3 | 0.4 ± 0 | 0.6 ± 0.1 | 0.7 ± 0.1 | 0.5 ± 0 | 0.2 ± 0.3 | 0.4 ± 0 | 0.8 ± 0.2 |
| 16:1n-7 | 1.4 ± 0.5 | 1.3 ± 0.5 | 1.2 ± 0.3 | 1.4 ± 0.4 | 1.8 ± 0.2 | 1.8 ± 0.1 | 1.4 ± 0.1 | 3.3 ± 2.1 | 2 ± 0.7 | 3.2 ± 0.9 |
| 16:2 | 0.4 ± 0.4 | 0.3 ± 0.4 | 0.4 ± 0.4 | 0.4 ± 0.5 | 0.3 ± 0.4 | 0.5 ± 0 | 0.2 ± 0.3 | 0.5 ± 0.5 | 0.6 ± 0.3 | 0.6 ± 0.1 |
| 16:2 | 0.6 ± 0.5 | 0.6 ± 0.3 | 0.6 ± 0.3 | 0.7 ± 0.6 | 0.6 ± 0.4 | 0.8 ± 0.3 | 0.4 ± 0.1 | 2.3 ± 2.7 | 1 ± 0.9 | 0.4 ± 0.2 |
| 16:3 | 0.4 ± 0.1 | 0.4 ± 0.2 | 0.4 ± 0 | 0.4 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.2 | 0.3 ± 0.2 | 2.4 ± 3.2 | 1.1 ± 0.7 | 0.6 ± 0.2 |
| 16:4 | 0.2 ± 0.2 | 0.2 ± 0.2 | 0.3 ± 0.2 | 0.4 ± 0.2 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.1 ± 0 | 0.9 ± 1.4 | 0.4 ± 0.4 | 0.2 ± 0.1 |
| 18:0 | 12.9 ± 2.7 | 12.0 ± 1.1 | 12.1 ± 1.2 | 11.3 ± 1.8 | 11.0 ± 0.6 | 11.5 ± 1.2 | 14.1 ± 0.2 | 16.5 ± 3.3 | 10.2 ± 5.7 | 9.3 ± 1.1 |
| 18:1n-9 | 14.2 ± 2.9 | 14.0 ± 1.4 | 15.2 ± 1.1 | 16.0 ± 2.6 | 17.0 ± 1.3 | 17.8 ± 1.0 | 16.1 ± 2.2 | 17.9 ± 2.9 | 17.8 ± 7 | 23.8 ± 4.3 |
| 18:1n-7 | 2.0 ± 0.4 | 2.1 ± 0.3 | 1.8 ± 0.2 | 1.9 ± 0.3 | 2.0 ± 0.2 | 1.9 ± 0.2 | 1.7 ± 0.2 | 2.1 ± 0.4 | 1.8 ± 0 | 2.9 ± 1.5 |
| 18:2n-6 | 7.5 ± 1.8 | 7.0 ± 1.1 | 7.4 ± 1.0 | 8.3 ± 1.7 | 8.2 ± 0.7 | 6.8 ± 0.9 | 5.8 ± 0.5 | 8.4 ± 5.1 | 7.5 ± 4.5 | 8.7 ± 5.4 |
| 18:3n-6 | 0.2 ± 0.1 | 0.2 ± 0 | 0.3 ± 0 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.2 | 0.2 ± 0.2 | 0.2 ± 0.2 |
| 18:3n-3 | 0.9 ± 0.4 | 0.7 ± 0.1 | 0.8 ± 0.2 | 0.9 ± 0.2 | 0.9 ± 0.2 | 0.7 ± 0.3 | 0.2 ± 0.1 | 1.3 ± 1.5 | 2.7 ± 2.9 | 0.8 ± 0.6 |
| 18:4n-3 | 0.5 ± 0.2 | 0.4 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.2 | 0.5 ± 0.1 | 0.5 ± 0 | 0.3 ± 0.1 | 1.3 ± 1.4 | 0.6 ± 0.2 | 0.6 ± 0.3 |
| 20:0 | 0.5 ± 0.5 | 0.3 ± 0.1 | 0.5 ± 0.2 | 0.4 ± 0.1 | 0.4 ± 0.2 | 0.1 ± 0.1 | 0.1 ± 0 | 0.1 ± 0.2 | 0.2 ± 0.1 | 0.6 ± 0.9 |
| 20:1 | 1.4 ± 0.1 | 1.3 ± 0.2 | 1.4 ± 0.2 | 1.2 ± 0 | 1.3 ± 0.3 | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.7 | 3.4 ± 1.7 | 1.4 ± 0.9 |
| 20:2 | 0.7 ± 0.2 | 0.6 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.1 | 0.6 ± 0.1 | 0.6 ± 0.1 | 0.6 ± 0 | 1.5 ± 1.8 | 0.9 ± 1.0 | 0.4 ± 0.1 |
| 20:3n-6 (DGLA) | 0.6 ± 0.1b | 0.5 ± 0.1b | 2.6 ± 0.4c | 4.0 ± 0.5a | 0.9 ± 0.3b | 1.2 ± 0.2 | 0.9 ± 0 | 2.6 ± 1.1 | 2.2 ± 0.4 | 0.7 ± 0.3 |
| 20:4n-6 (ARA) | 8.8 ± 1.1c | 11.3 ± 0.7a | 3.4 ± 0.1b | 3.4 ± 0.6b | 2.2 ± 0.4b | 6.9 ± 1.8 | 11.3 ± 0.7 | 1.2 ± 0.8 | 2.8 ± 2.6 | 1.4 ± 1.0 |
| 20:3n-3 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.2 | 0.2 ± 0.1 | 0.2 ± 0 | 0.3 ± 0.3 | 0.1 ± 0.1 | 0.5 ± 0.7 | 0.5 ± 0.5 | 0.2 ± 0.1 |
| 20:4n-3 | 0.5 ± 0.1 | 0.3 ± 0.1 | 0.6 ± 0.6 | 0.4 ± 0 | 0.4 ± 0 | 0.2 ± 0.1 | 0.2 ± 0 | 0.5 ± 0.6 | 0.2 ± 0.2 | 0.3 ± 0.1 |
| 20:5n-3 (EPA) | 2.9 ± 0.5b | 2.4 ± 0.3b | 3.9 ± 0.6a | 3.5 ± 0.4a | 4.1 ± 0.8a | 2.8 ± 0.5 | 2.0 ± 0.4 | 1.0 ± 0.5 | 1.9 ± 1.5 | 2.5 ± 1.2 |
| 22:0 | 0.4 ± 0.3 | 0.3 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.4 ± 0.2 | 0.1 ± 0.1 | 0.3 ± 0.3 | 1.6 ± 2.1 | 0.8 ± 1.1 |
| 22:4n-6 | 0.6 ± 0.1ab | 1.0 ± 0.3a | 0.4 ± 0.1b | 0.3 ± 0.1b | 0.4 ± 0.1b | 0.6 ± 0.2 | 0.8 ± 0 | 0.1 ± 0.1 | 2.5 ± 0.1 | 0.4 ± 0.2 |
| 22:5n-6 | 0.5 ± 0.2 | 0.5 ± 0.2 | 0.6 ± 0.3 | 0.5 ± 0.3 | 0.5 ± 0.3 | 0.4 ± 0 | 0.5 ± 0.1 | 0 ± 0 | 0.9 ± 1.0 | 0.3 ± 0.2 |
| 22:5n3 | 1.2 ± 0.2 | 1.1 ± 0.2 | 1.2 ± 0.1 | 1.2 ± 0.3 | 1.4 ± 0.2 | 1.5 ± 0.3 | 1.3 ± 0.3 | 1.9 ± 1.8 | 0.6 ± 0.6 | 1.1 ± 0.4 |
| 22:6n3 | 16.3 ± 1.7 | 17 ± 1.9 | 19.2 ± 2.0 | 17.0 ± 3.0 | 19.6 ± 3.3 | 16.8 ± 0.7 | 17.4 ± 1.6 | 3.9 ± 4.2 | 5.2 ± 5.5 | 8.9 ± 6.4 |
| FA content (μg mg−1 DW)* | ||||||||||
| DGLA | 0.2 ± 0.1b | 0.3 ± 0.2b | 0.7 ± 0.2b | 1.7 ± 0.9ab | 0.4 ± 0.1a | 0.2 ± 0b | 0.1 ± 0b | 0.6 ± 0.3a | 0.4 ± 0ab | 0.1 ± 0b |
| ARA | 2.8 ± 0.8ab | 5.3 ± 3.0a | 1.0 ± 0.4b | 1.5 ± 0.9b | 0.9 ± 0.2b | 1.0 ± 0.2a | 1.6 ± 0.4a | 0.2 ± 0.2b | 0.3 ± 0b | 0.2 ± 0.1b |
| TFA | 32.9 ± 9.2 | 47.2 ± 26.9 | 28.9 ± 11 | 42.6 ± 21.4 | 39.2 ± 6.1 | 15.4 ± 2.4 | 14.1 ± 2.6 | 22 ± 1.6 | 15.3 ± 0 | 16.1 ± 9.2 |
Data represents fatty acid composition from the polar fraction before and after S. iniae challenge. Values shown as mean ± SD, n = 20. Different letters a, b and c denote significant (p < 0.05) difference in treatments, as determined by ANOVA.
*
FA content represents μg of the FA per mg of liver dry weight (DW).