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. 2018 Aug 30;11(3):770–783. doi: 10.1016/j.stemcr.2018.08.006

Figure 2.

Figure 2

HOPX Exhibits a Complex Spatial and Temporal Expression Pattern within the SVZ Where it Labels a Subpopulation of dNSCs

(A) Representative micrographs of HOPX expression in coronal sections at E16, P1, P4, P21, and P60 (top panels). Higher magnifications (lower panels) show high expression in the embryo (E16) and at early postnatal stages (P1, P4) and a decline thereafter (P21, P60). A clear medial-to-lateral HOPX expression gradient is apparent in the dSVZ (see also Figure S2 for a more complete rostrocaudal overview at P4).

(B and C) Analysis of HOPX expression in dNSCs was performed in HES5:EGFP mice (B; n = 3 animals) and after short-term (8 hr) targeted EPO of a pCX-GFP plasmid (C; n = 6 animals). Crops show overlap of HOPX with HES5:EGFP- and GFP-positive cells, respectively, which was more pronounced in the medial than lateral subdomain of the dSVZ (crops and graphs).

EPO, electroporation; dSVZ, dorsal SVZ; lSVZ, lateral SVZ; dNSC, dorsal NSC. Error bars represent the SEM. Scale bars in (A) represent 1 mm (overview) and 500 μm (crops). Scale bars in (B) and (C) represent 200 μm (DAPI overview), 100 μm (higher magnification), and 25 μm (crops). ∗p < 0.05;∗∗∗p < 0.001.