Figure 2.

Vesicular Glutamate Transporter VGlut Localizes to Axon Terminals of L1, Mi9, and LPi4-3 Neurons Indicating their Glutamatergic Phenotype

(A–C) Upper rows show overviews of optic lobes with L1 (A), Mi9 (B), and LPi4-3 (C) labeled with myr::GFP (green), background staining against bruchpilot brp (gray), and anti-VGlut staining (magenta). In the lower rows higher magnifications of axon terminals of L1, Mi9, and LPi4-3 neurons are depicted (sections marked with white boxes in overview images).

(A) L1 axon terminals in medulla layers 1 and 5 show overlapping signal with anti-VGlut staining.

(B) VGlut protein co-localizes with Mi9 axons in layer 10 of the medulla.

(C) Lobula plate intrinsic neurons LPi4-3 have their dendrites in layer 4 and project their terminals to layer 3. Labeled with arrowheads are LPi boutons in layer 3 showing overlapping signal with anti-VGlut staining. Shown here are single planes of confocal stacks. Scale bar for overview of optic lobes is 20 μm. For higher magnification close-ups the scale is 5 μm. White dashed lines in the lower panel are manually drawn and indicate layers of the lobula plate.