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Microfluidic motile sperm sorter Qualis® is composed of four chambers named as Chamber [1], [2], [3], [4] and a micro channel being connected to each chamber. After applying sperm sorting medium to Chamber [2], [3], [4] and semen sample to Chamber [1] at an appropriate amount, two streams of fluid with laminar flow can be formed in parallel ([1]→[4], [2]→[3]). Only motile spermatozoa are able to swim into the parallel stream, after which they are isolated in Chamber [3].
