Table 2.

Summary of the Rigidity-Based Allosteric Analyses

Parameters	Nitrate-Unbound Protomer A [30, 94]	Nitrate-Bound Protomer A [30, 94]
Rigid clusters	[32, 52], [55, 64], [67, 69], [75, 94]	[30, 94]
No. of H-bonds	46	49
H-bonds broken in [30, 94] after $\mathit{N}{\mathit{O}}_3^{-}$ binding	Ala32-Met36, Ser33-Ile37, Met36-Cys39, Glu44-Thr47, Arg45-Leu49, Thr48-Gly52, Thr57-Thr60, Tyr58-Thr62, Leu59-Leu65, Ala70-Thr73, Asn72-Thr75, Ile91-Thr94
Newly added H-bonds in [30, 94] after $\mathit{N}{\mathit{O}}_3^{-}$ binding	Gly30-Ser33, Ile37-Cys39, Val43-Thr47, Glu44-Arg45, Glu44-Thr48, Asn54-Val56, Thr69-Thr73, Ala71-Val74, Ala71-Thr75, Phe77-Ser81, Leu78-Phe82, Ser81-Cys85, Leu86-Phe90, Phe90-Thr94, Phe90-Thr94
No. of H-bonds conserved in [30, 94] before and after $\mathit{N}{\mathit{O}}_3^{-}$ binding	34