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. 2018 Feb 15;1:1–15. doi: 10.1016/j.isci.2018.01.003

Figure 1.

Figure 1

Potentiation of the Transcriptional Co-activator Activity of YAP and TAZ toward TEAD by Parafibromin

(A) HEK293T cells were transiently transfected with a TEAD luciferase reporter together with the indicated plasmids. Total cell lysates were subjected to luciferase assay and immunoblotting with indicated antibodies. PF, Parafibromin.

(B) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of Cyr61 and Ctgf mRNA expression in HEK293T cells transiently transfected with a PF or control empty vector (Con).

(C and D) HEK293T cells were transiently transfected with a TEAD luciferase reporter together with indicated plasmids. Total cell lysates were subjected to luciferase assay and immunoblotting with indicated antibodies.

(E) HEK293T cells were transiently transfected with indicated plasmids. Total cell lysates were subjected to luciferase assay and immunoblotting with indicated antibodies.

(F) qRT-PCR analysis of Cyr61 and Ctgf mRNA expression in HEK293T cells transiently transfected with sgPF vector or a control vector.

Error bars, mean ± SD; n = 3; **p < 0.01 versus control vector (A–F), YAP1α with control vector (C), YAP2δ with control vector (C and E), or TAZ with control vector (D and E); ‡p < 0.01 versus YAP1α with PF (C).

See also Figure S1.