Figure 4.
Physical Interaction between Parafibromin and YAP/TAZ
(A) HEK293T cells were transfected with indicated plasmids. Total cell lysates (TCLs) and anti-FLAG immunoprecipitates (IPs) from TCLs were analyzed by immunoblotting with anti-FLAG and anti-HA antibodies.
(B) An anti-PF (left) or anti-YAP/TAZ (right) immunoprecipitate from HEK293T cell lysates was analyzed by immunoblotting with anti-PF and anti-YAP/TAZ antibodies. Rabbit IgG was used for a negative control antibody of immunoprecipitation.
(C and D) HEK293T cells were transiently transfected with indicated plasmids. TCLs and anti-FLAG IPs from TCLs were analyzed by immunoblotting with anti-FLAG and anti-HA antibodies.
(E and F) HEK293T cells were transiently transfected with a TEAD luciferase reporter together with indicated plasmids. TCLs were subjected to luciferase assay and immunoblotting with the indicated antibodies.
(G) HEK293T cells were transiently transfected with indicated plasmids. TCLs and anti-FLAG IPs from TCLs were analyzed by immunoblotting with anti-FLAG and anti-HA antibodies.
(H) HEK293T cells were transiently transfected with a TEAD luciferase reporter together with indicated plasmids. TCLs were subjected to luciferase assay and immunoblotting with the indicated antibodies.
Error bars, mean ± SD; n = 3; **p < 0.01 versus YAP2δ with PF (E), control vector (F and H), PF with control vector (F), YAP2δ with control vector (H), or YAP2δmWW2 with control vector (H); ‡p < 0.01 versus TAZ with control vector (F), TAZ with PF (F), or PF with YAP2δ (H).
See also Figure S4.