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. 2018 Feb 15;1:1–15. doi: 10.1016/j.isci.2018.01.003

Figure 6.

Figure 6

Inverse Regulation of YAP and TAZ Functions by Tyrosine Phosphorylation/Dephosphorylation of Parafibromin

(A) HEK293T cells were transfected with indicated plasmids. TCLs were subjected to a sequential immunoprecipitation-immunoblotting analysis with indicated antibodies (left) and luciferase assay (right).

(B) HEK293T cells were transiently transfected with a TEAD luciferase reporter together with indicated plasmids. The cell lysates were subjected to luciferase assay.

(C) HEK293T cells were transfected with indicated plasmids. Anti-FLAG IP from cell lysates and TCL were subjected to immunoblotting with indicated antibodies.

(D) HEK293T cells were transiently transfected with a TEAD luciferase reporter together with indicated plasmids. The cell lysates were subjected to luciferase assay.

(E and F) HEK293T cells were transfected with indicated plasmids. TCLs and anti-FLAG immunoprecipitates from TCLs were subjected to immunoblotting with indicated antibodies.

Error bars, mean ± SD; n = 3; **p < 0.01 versus control vector (A and B), control vector with YAP2δ (D), or control vector with TAZ (D); ‡p < 0.01 versus PF with TAZ (A and D), or PF with YAP2δ (B).

See also Figure S6.