. 2018 Sep 12;3(5):e00286-18. doi: 10.1128/mSphere.00286-18

TABLE 1.

Assessment of differentially expressed proteins of NTHI transiently restricted and continuously exposed to heme-iron^a

NCBI protein GI	NTHI no.	Gene	Annotation	Protein description	Peptide no.	Fold change	P value
GI:895836 505	NTHI0175		Conserved hypothetical	Class I SAM-dependent methyltransferase	6	6	0.0005
GI:915525 770	NTHI1895	tehB	Tellurite resistance protein	Tellurite resistance methyltransferase TehB	5	4.5	0.0034
GI:100606 0856	NTHI0351	queA	S-Adenosylmethionine:tRNA ribosyltransferase-isomerase	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	4	3.1	0.003
GI:499591 591	NTHI1169	tbp2	Transferrin-binding protein 2	Transferrin-binding protein-like solute binding protein	8	3	0.0075
GI:501001 680	NTHI0034	lipB	Lipoate-protein ligase B	Lipoyl(octanoyl) transferase LipB	3	2.5	0.0048
GI:764356 334	NTHI0605	hisF	Imidazole glycerol phosphate synthase subunit HisF	Imidazole glycerol phosphate synthase subunit HisF	3	1.9	0.009
GI:915492 423	NTHI1899		Conserved hypothetical protein	30S ribosomal protein S12 methylthiotransferase accessory protein YcaO	4	1.8	0.0031
GI:499591 843	NTHI1649	fabA	3-Hydroxydecanoyl-[ACP] dehydratase	Beta-hydroxydecanoyl-ACP dehydratase	9	1.6	0.0078
GI:915560 482	NTHI1099	hktE	Catalase	Catalase	2	−2.2	0.0098
GI:491953 725	NTHI1002	frdA	Fumarate reductase flavoprotein subunit	Fumarate reductase (quinol) flavoprotein subunit	15	−2.4	0.002
GI:499591 611	NTHI1207	dmsA	Anaerobic dimethyl sulfoxide reductase chain A precursor	Dimethyl sulfoxide reductase subunit A	9	−2.9	0.0002
GI:100603 3258	NTHI1000	frdB	Fumarate reductase iron-sulfur protein	Succinate dehydrogenase/fumarate reductase iron-sulfur subunit	6	−3.1	0.0045
LIPA_HA ES1*	NTHI0033	lipA	Lipoic acid synthetase	Lipoyl synthase OS = Haemophilus somnus	2	−3.2	0.0092
GI:100603 3084	NTHI1236	cyoA	Probable cytochrome oxidase subunit I	Cytochrome d terminal oxidase subunit 1	6	−4.2	0.0025
GI:116877 7694	NTHI1230	nrfA	Cytochrome c₅₅₂	Ammonia-forming nitrite reductase cytochrome c₅₅₂ subunit	11	−20.1	0.0027

LC-MS/MS was performed on three independent biological replicates of 24-h planktonic cultures of transiently restricted (TR) or continuously exposed (CE) NTHI. A total of 6,973 peptides and 1,183 proteins were quantified. Proteins represented with a single peptide count were excluded. Of the remaining proteins, those which passed a fold change cutoff of ±1.5 and P < 0.01 are included in the table. Differential expression determination was based on calculation of the fold change (positive numbers indicate proteins that are more represented in the TR population; negative numbers indicate proteins that are more represented in the CE population), as determined by a two-tailed Student t test. ACP, acyl carrier protein.