TABLE 2.
Primers used for RT-PCRs and sequencing
| Primer | Primer sequence (5′–3')a | Orientation | Location (nt)b |
|---|---|---|---|
| 8-APN35 | GAGAAAXGGGACGTCXGCGC | Forward | 522 |
| 8-APN2 | GTCXCCTATTCAGGCXTAGAAG | Reverse | 990 |
| 8-APN3 | GGCTAAGGATGCCCTTCAG | Forward | 894 |
| 9-XPN18 | TTXGAXAACCAXTCXTTXTTXTGXGTGTT | Reverse | 1832 |
| 14-CPN63 | CCGTTGGAGGTGACACACG | Reverse | 1503 |
| 14-CPN7 | ATGCCATCAGTGGAGGCTCC | Forward | 1773 |
| 14-CPN6 | GTCCAACAGGTTGGTGAAGC | Reverse | 2679 |
| 14-CPN5 | ATGGCAAGGTGTACAACCCG | Forward | 2637 |
| 11-FPN35 | GAARGGCCCRGGGTTGGAC | Reverse | 3898 |
| 14-CPN61 | GGAGGCGCAACTCAAAGTC | Reverse | 3185 |
| 14-CPN3 | TACAACAAGGCACCATTCACG | Forward | 3542 |
| 11-SPN3 | ACACTGTCGCCAGCACACG | Reverse | 3593 |
| 14-CPN4 | CCAGACCGCTGTTGGCAATAG | Forward | 3783 |
| 8-APN45 | GGAAGAAACTCGAGGCGAC | Reverse | 4316 |
| 8-APN22 | AAGGACCCXGTCCTTGTGGC | Forward | 4151 |
| 1-XPN28 | GTTGTAGCCGTCXAAGTGGTC | Reverse | 4808 |
| 8-APN46 | TGGTCGTTTGCCTCCGTGG | Forward | 4683 |
| 8-APN87 | CTCAAAGAATTCAATTGCTGC | Reverse | 5387 |
| 8-APN13 | GCXCTTCTXAACGGXATGGC | Forward | 5171 |
| 8-APN68 | GGGTCCTTCAGCTGGTGG | Reverse | 5774 |
| 8-APN113 | CGCGAXACTCGCAAGAGAC | Forward | 5555 |
| 9-XPN11 | AGCATGTCCTGTCCTTTTACT | Reverse | 6223 |
| 14-CPN2 | CCATTTGCTGTGCTACTGGA | Forward | 6084 |
| 8-APN114 | CAGGGTTGAACACACCGTG | Reverse | 6716 |
| 9-XPN2 | AATGAAGGCACACXTXGAXCCXGA | Forward | 6601 |
| 14-CPN1 | AGGGTTACAACCGACCGCG | Reverse | 7276 |
| 8-APN17 | CTGAAGGACGAXXTXCGXCC | Forward | 7124 |
| 8-APN52 | GGAXTGACCAAGAACAAAACC | Reverse | 7754 |
| 9-XPN23 | TGGACACXTACACCATGATCTC | Forward | 7620 |
| NVT27 | TTTTTTTTTTTTTTTTTTTTTTTTTTTVN | Reverse | 8140 |
a
X = inosine.
b
The primer locations are based on the sequence of FMDV A24 Cruzeiro (accession no. AY593768.1). For forward primers, the 5′-terminal nucleotide (nt) is used, while for the reverse primers, the position of the nt that is complementary to the 3′ nt of the primer is given.