Figure 10.

Clonal spheres are highly tumorigenic. The D3 clonal spheres were dissociated into single cells and freshly sorted Aldefluor (ALD)⁺ THJ-16T cells were injected s.c. into nude mice at 1 × 10⁵ cells per injection site (3 injection sites, one in each flank and one in the back). A: The D3 clone forms larger tumors faster than the ALD⁺ tumors. B: RT-PCR results comparing stem cell marker expression of the D3 tumors and the ALD⁺ tumors. ^∗P < 0.05, ^∗∗P < 0.01 versus respective ALD⁺ tumor.