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. 2016 Jun;186(6):1662–1673. doi: 10.1016/j.ajpath.2016.02.003

Figure 7.

Figure 7

Clonal sphere enriched for stem cell markers. A: Flow cytometric analysis of Aldefluor positivity with or without DEAB (aldehyde dehydrogenase inhibitor) of D3 clone. B: Graph indicating reversal of the percentage of Aldefluor (ALD) positive versus negative cells in the parental THJ-16T cell line compared with the D3 clone. C: RT-PCR results of stem cell markers comparing the parental THJ-16T cell line with freshly sorted ALD+ cells and the D3 clonal line. D: Percentage of Aldefluor and stage-specific embryonic antigen 1 (SSEA1) positivity of the D3 and D6 clonal lines compared with the parental THJ-16T cell line. ∗∗P < 0.01, and ∗∗∗P < 0.001 versus ALD−. †P < 0.05, ††P < 0.01, and †††P < 0.001 versus the 16T parental line control group.