Table 2.

Analysis of Thyroid Carcinoma Cell Lines by Flow Cytometry for Aldefluor Activity and SSEA1 Expression

Cell line	ALD (%)	SSEA1 (%)	Spheres % (no./total)
16T	4.1 ± 1.7	5.3 ± 1.02∗	68 (81/120)
21T	2.4 ± 0.2	3.16 ± 0.23	25 (3/12)
FRO	3.5 ± 1.5	0.59 ± 0.41	5 (6/120)
KAT18	2.4 ± 0.2	0.85 ± 0.56	4 (2/48)
TPC1	0.1 ± 0.03	0.07 ± 0.04	25 (3/12)
BCPAP	0.5 ± 0.1	1.99 ± 0.09	58 (7/12)

ALD and SSEA1 expressed as means ± SEM. Each line was also analyzed for thyrosphere formation efficiency, (x, y) where x is the number of spheres formed, and y is the number of wells tested.

ALD, Aldefluor; SSEA1, stage-specific embryonic antigen 1.

^∗P < 0.05.