Figure 2. Karyotypes of Characidium females subjected to fluorescence in situ hybridization (FISH) with TE probes. (a) C. zebra, (b) C. gomesi (PG), (c) C. gomesi (SJ); (d) C. zebra, (e) C. gomesi (SJ). The Rex3 probe did not show any hybridization signals in C. gomesi (PG) chromosomes (not shown). The W and Z sex chromosomes of C. gomesi females are highlighted in the box. PG, Paiol Grande Stream population; SJ, São João River population. Scale bar, 10 μm.