Figure 5. PCR analysis of PfHSP17.2 transgenic plants and stress tolerance tests. (a) Semi-quantitative RT-PCR analysis of PfHSP17.2 expression in WT and PfHSP17.2 transgenic plants grown at 22°C for 4 weeks; (b) Heat treatments: aquired heat tolerance and basal heat tolerance; (c) 3-week-old plants heat treatment comparison; (d) survival rate after acquired heat treatment; (e) effect of heat stress on hypocotyl elongation of WT and PfHSP17.2-overexpressing plants; (f) MDA; (g) electrolyte leakage. For survival rate (d), hypocotyl elongation (e), MDA (f) and electrolyte leakage (g), error bars indicate SD (n=3). *p<0.05, ** p<0.01. WT, wild type; L223, L253, two transgenic Arabidopsis lines.
